The match in Durban on Sunday morning (AEST) pits the sixth-placed Sharks against a Brumbies team languishing in 10th spot with just two wins from nine games.
The Brumbies are coming off a 47-36 loss to the Cheetahs in Bloemfontein in which they conceded three tries in the first 12 minutes to be 25-0 down.
"We spoke last week about having a fast start and we let ourselves down," Brumbies lock Mark Chisholm told AAP from Durban.
"We've really got to aim up this week and the start is the major thing we're concentrating on."
The Cheetahs kicked five penalties in the Bloemfontein fixture, but Chisholm denied discipline had been a major issue for his team.
"I don't think discipline was an issue. I think it was more the honesty with ourselves and the trust within our defensive line let us down," Chisholm said.
"Anyone who watched that game would have seen it. There were so many defensive errors. That can't happen at the start of a game."
Chisholm recognised the high difficulty factor of beating the Sharks on their own ground.
"They are always tough down at the Shark Tank. They've got a big following here and they and always rise to the occasion," Chisholm said.
The Sharks will be without two injured forwards, captain and front rower John Smit and No.8 Ryan Kankowski.
Coach Tony Rea made a number of changes to the Brumbies team.
Playmakers Matt Giteau and Christian Lealiifano have swapped jerseys, with the former listed to line up at five-eighth and the latter at inside centre.
Tyrone Smith will make his first start of the year at outside centre, with Andrew Smith shifting to the wing to replace Francis Fainifo, who drops to the bench.
No.8 Ita Vaea returns from injury, with Colby Faingaa switching to openside flanker in place of Michael Hooper, who is relegated to the reserves.
Chisholm predicted a bright future for prop Jono Owen, who will make his run-on debut in place of the injured Dan Palmer.
"He's got a big future ahead, he's pretty excited about getting his first start and I'm pretty sure he'll prove that he should be in the squad fulltime," Chisholm said.
"It is the Canberra-based team's first match in Durban since the death there two years ago of Brumbies forward Shawn Mackay.
"That happened a long time ago and Shawn is definitely still with us, in our minds," Chisholm said.